V. V. Tuchin, Tissue Optics and Tissue Optical Clearing for in-depth Imaging

V. V. Tuchin (Optics and Biophotonics Chair, Saratov State University)
Tissue Optics and Tissue Optical Clearing for in-depth Imaging

A brief description of tissue optics, concept of ‘tissue optical windows’ and method of optical clearing (OC) based on controllable and reversible modification of tissue optical properties by their impregnation with a biocompatible optical clearing agent (OCA) will be done. Fundamentals and major mechanisms of OC allowing one to enhance optical imaging facilities and laser treatment efficiency of living tissues will be presented. The enhancement of probing/treatment depth and image contrast for a number of human and animal tissues investigated by using different optical modalities, including diffuse reflectance spectroscopy, collimated transmittance, OCT, photoacoustic microscopy, linear and nonlinear fluorescence, SHG and Raman microscopies will be discussed. Experimental data on the diffusion and permeability coefficients of biocompatible FDA approved OCAs, such as glucose, glycerol, PEG, albumin, CT contrast agents (Iohexol (OmnipaqueTM) and Iodixanol (VisipaqueTM)), and MRI contrast agents (Gadobutrol (GadovistTM)) in normal and pathological tissues (cancer and diabetes) will be presented. Perspectives of immersion optical clearing/contrasting technique aiming to enhance imaging of living tissues by using different imaging modalities working in the ultra-broad wavelength range will be discussed.

References:

1. D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Recent progress in tissue optical clearing, Laser Photonics Rev. 7(5), 732–757 (2013).

2. E. A. Genina, A. N. Bashkatov, Yu. P. Sinichkin, I. Yu. Yanina, V.V. Tuchin, “Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy,” J. Biomed. Photonics & Eng. 1(1), 22–58 (2015).

3. V. V. Tuchin, Polarized light interaction with tissues, J. Biomed. Opt. 21(7), 071114-1-37 (2016).

4. A.Yu. Sdobnov, M.E. Darvin, E.A. Genina, A.N. Bashkatov, J. Lademann, V.V. Tuchin, Recent progress in tissue optical clearing for spectroscopic application, Spectroch. Acta Part A: Molec. & Biomol. Spectrosc. 197, 216–229 (2018).

5. O.A. Smolyanskaya, et al., “Terahertz biophotonics as a tool for studies of dielectric and spectral properties of biological tissues and liquids,” Progress in Quantum Electronics 62, Nov. 1-77 (2018).

6. A. N. Bashkatov, et al., “Measurement of tissue optical properties in the context of tissue optical clearing,” J. Biomed. Opt. 23(9), 091416 (2018).

7. D. K. Tuchina and V. V. Tuchin, Optical and structural properties of biological tissues under diabetes mellitus, J. Biomed. Photonics & Eng. 4 (2) 020201-1-22 (2018).

8. L. Oliveira and V. V. Tuchin, The Optical Clearing Method: A new tool for Clinical Practice and Biomedical Engineering, Basel: Springer Nature Switzerland AG, 2019.

9. D.K. Tuchina, I.G. Meerovich, O.A. Sindeeva, V. V. Zherdeva, A. P. Savitsky, A. A. Bogdanov Jr, V. V. Tuchin, Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging. J. Biophotonics 13(11), e201960249 (2020).

10. I. Carneiro, S. Carvalho, R. Henrique, A. Selifonov, L. Oliveira, V.V. Tuchin, Enhanced ultraviolet spectroscopy by optical clearing for biomedical applications, IEEE Journal of Selected Topics in Quantum Electronics 27 (4), 1-8 (2021).

Slides are availible here:
https://drive.google.com/file/d/10medCU6MUtWp1gE038cIUOLh409VZ6Fd/view?usp=sharing